Fish-less Cycling Program: Everything You Need to Know About Cycling New Jellyfish Aquariums

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***At this time, we are suspending the “program” part of our Fish-less Cycling Program due to reduced staff availability. Signups will not be accepted until further notice. If you are setting up a new jellyfish aquarium, we still strongly recommend you still cycle it using the fish-less method before introducing your first jellyfish.***


Thanks for your interest in our Fish-less Cycling Program! This program was set up back in 2016 for two reasons: 1) to help new jellyfish owners have a successful start with their new aquariums, and 2) clear up misinformation floating around the internet about the cycling process and how long it takes to successfully cycle a new aquarium. Cycling a new aquarium takes time, patience, and attention to detail but it ensures your new aquarium is biologically stable and habitable for any saltwater animal; this is not jellyfish-specific issue! This blog post provides the essential information you need to know about what cycling is and how to cycle your new aquarium. At the bottom of this post (after you’ve read every bit of information and become a cycling expert, of course!), you’ll find the sign up form to participate in our Fish-less Cycling Program.

A “fish-less cycle” is the best and safest way to cycle an aquarium, as it doesn’t harm any fish or jellyfish. The goal of cycling a new tank is to establish a healthy and strong colony of beneficial bacteria in the filters to take care of waste that is created by feeding the animals (in this case, jellyfish!).  The process of cycling a new aquarium is often overlooked and can create problem right from the get-go. New jellyfish owners who do not allow their tank to completely cycle before adding their first jellyfish sadly end up with unhealthy & deformed jellyfish and become confused about why they are not well. Since jellyfish are 96% water, they rely heavily on water chemistry (a.k.a. water quality) and therefore, need a biologically stable and “chemically clean” environment in order to thrive.

Cycling takes no less than 4 weeks. There is misinformation going around the Internet about how long cycling takes. Some websites claim “it only takes 10-14 days” or “two weeks.” This is wrong.  The nitrogen cycle is straight forward biology and you can’t speed up biology.  Even with the use of “bio-starters,” you must still cycle the aquarium and it will still take 4 weeks minimum.


 Now, let’s get to what it actually means to cycle your  new aquarium!

Cycling is turning a static, non-living environment (i.e. brand new tank/filters) into a biologically stable environment that’s safe for live animals.

What do we mean when we keep saying, “biologically stable environment”? Well, the filters (i.e. bioballs, rock media, etc.) of an aquarium need to have strong, healthy colonies of beneficial bacteria established within them. Beneficial bacteria = good bacteria! They take care of the waste created from daily feedings. Cycling allows these bacteria to establish themselves by multiplying and creating large colonies able to handle the waste created within your aquarium by the fish and the food being fed daily.

The Nitrogen Cycle: This is a three-step process converting ammonia (NH₃) into nitrite (NO₂) and finally into nitrate (NO₃)– all done through the bacteria. The “cycling process” is essentially the “nitrogen cycle”! All three of these compounds will be present at some point during cycling and since ammonia and nitrite are toxic to all saltwater animals (not just jellyfish), this is why cycling should be done “fish-less” and before introducing the first animals. Nitrate is a by-product of the nitrogen cycle that will always be present in your aquarium and is not toxic to moon jellyfish under 100 ppm; so, no need to worry about nitrate while cycling! It is always maintained through weekly water changes once cycling is done.

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Graph estimating how the nitrogen cycle progresses over time. Taken from “The Nitrogen Cycle” by Centreville Aquarium Centre.

Fish-less cycling involves placing a nickel-sized (approx. ¾” x ¾”) piece of raw shrimp (add one nickel-sized shrimp piece for every 10g of water volume your aquarium can hold) into the filter box or compartment of your aquarium. The raw shrimp will begin to decompose and create ammonia to start the cycling process. At the same time, a starter bacteria culture is added to begin the colonization in the filters. After about two weeks, the ammonia level will rise to a peak (also known as “spiking”) and then start to decrease to zero. Once the ammonia is finished spiking, the nitrite level will begin to rise. After another two weeks, the ammonia will return to zero and the nitrite won’t be far behind. Once the ammonia and nitrite levels both return to zero, the aquarium is cycled!


Let’s move onto the

Fish-less Cycling Program!

 Here’s what you’ll need to start:

  • Freshly mixed or store bought saltwater
  • Bacteria source – we recommend using BioSpira [Purchase from Amazon here]
  • Ammonia source – a nickel-sized piece (¾” x ¾”) of a raw shrimp for every 10g saltwater
    • Single shrimp can be bought from the fresh seafood section of your local grocery store
  • API Saltwater Master Test Kit – includes pH, NH3, NO2, NO3 [Purchase from Amazon here]
  • Thermometer and hydrometer or refractometer – to test for temperature and salinity
  • Fish-less Cycling Program Guidelines – this is your weekly guide to see what the *expected* week-by-week changes in water quality (also a.k.a. the nitrogen cycle) for your tank [download the PDF below]
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Screenshot taken from our EON Instructional Video Series video about cycling. Watch the video here.

Let’s start cycling!

  1. Once your new aquarium is set up and running with new saltwater (salinity should be 1.022-1.024 Specific Gravity or 31-33ppt), add the bacteria source and two nickel-sized pieces of shrimp at the same time.
  2. Take your baseline water quality readings – this includes temperature, salinity, pH, ammonia, and nitrite.
  3. Test for pH, ammonia, and nitrite every week to keep track of the fluctuations in levels that indicate where you are in the cycle. Record these on the PDF guideline water quality sheet (download below).
  4. Once both ammonia and nitrite have returned to zero after subsequent spikes, your aquarium is ready for jellyfish! (Keep the piece of shrimp in your aquarium until the jellyfish arrive. It will continue to feed the newly established beneficial bacteria until you begin a daily feeding routine with jellyfish).

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Click to download the Fish-less Cycling Program Guidelines PDF.


DO’s & DON’T’s of Cycling

  • DO read this entire blog post and the Fish-less Cycling Program Guidelines to fully understand the cycling process and how to cycle your new jellyfish aquarium
  • DON’T skip any part of this blog as it contains all of the essential information you need to know about cycling!
  • DO use new saltwater with the following baseline water quality parameters:
    • Temperature = 65-78°F
    • Salinity = 31-33 ppt or 1.023-24 SG
    • pH = 8.0-8.1
  • DON’T add any buffers, conditioners, or additives to your aquarium water before or during the cycle – these can throw off the water quality and disrupt or inhibit the cycle from starting
  • DO use Instant Ocean Sea Salt mix for your saltwater as it has no buffers, conditioners, or additives [Purchase from Amazon here]
  • DON’T use any salt mix labeled as “reef salt,” “pH balanced,” “probiotic,” or “enriched” as these tends to have extra minerals, vitamins, and higher levels of pH specific for corals, anemones, etc. in reef tanks
  • DO make sure your pump is plugged in, turned on, and working properly
  • DON’T keep your tank near windows and/or in direct sunlight to keep algal growth at a minimum
  • DO use freshwater labeled only as “Distilled Water”
  • DON’T use freshwater jugs labeled “Spring Water,” “Purified Water,” or anything else to mix your saltwater or for topping off
  • DO add distilled/RO water to compensate for evaporation (evaporation causes salinity to rise; adding freshwater helps to bring it back down)
    • Evaporation is noticeable when the water level is lower than where it started
  • DON’T add distilled/RO water near the filters – this can completely wipe out your growing bacteria colonies
    • Only add freshwater to the main viewing area
  • DO record your weekly test vial readings via pictures with the color chart
  • DON’T use test strips as they often give unreliable readings
  • DO start the cycling process with a newly setup aquarium.
    • If your aquarium has been running with saltwater & the bio starter bacteria for longer than a week, you will need to dump the water and start over with all new saltwater & new bacteria.
  • DON’T perform any water or filter changes during cycling – this can disturb the growing bacteria and disrupt the cycle
  • DO keep an eye on the piece of shrimp in your tank – sometimes it can completely disintegrate before the cycle is over and will need to be replaced to keep the ammonia production up
  • DON’T freak out if your cycle isn’t exactly on track – every cycle is different and sometimes an aquarium needs an extra week to catch up
  • DON’T add a bubbler if you own a Cubic Orbit 20 jellyfish aquarium before or during the cycle – aeration can inhibit the cycle from starting

Things that can delay or disrupt the cycling process

  • Starting with a pH higher than 8.2 can inhibit the cycling process from starting by disturbing the bacteria. The pH of an aquarium will naturally drop during the cycle – which is normal! – and needs to be left alone to do its thing until the cycle is done.
  • Starting with salinity too high or too low can also inhibit the cycling process from starting by disturbing the bacteria. The optimal salinity range for moon jellyfish is 31-33 ppt or 1.023-24 SG, which is perfect for cycling.

Problem with Your Jellyfish? First Order of Business… Salinity!

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I recently had the opportunity to meet and speak at length with some new local customers who came in to learn how to keep jellyfish as pets.  Payam, Lauren and their little dog Ted came down to the lab to see just what is involved with keeping jellyfish and maintaining a jellyfish aquarium.  It was super to spend time with them and show them the ropes!  It was then that I realized that most of the questions they had are the same questions a lot of folks send my way via email. So, I will start to shoot quick blog posts every week that cover these questions– most of which are not jellyfish specific questions, but standard aquatics questions that can be answered fairly easily and quickly.  And, I will certainly get to the more specific jellyfish inquiries which will help you, as a jellyfish owner, begin to recognize and identify certain issues that can arise while caring for these beautiful animals and how to correct the problem right away.

Don’t bog your jellies down with high salinity levels in your jellyfish aquarium!

The first and biggest problem I see is maintaining water quality–specifically salinity.  The problem seems to be in the hydrometers that are generally purchased to measure the salinity. The plastic hydrometers that are on the market, and even the more costly refractometers need calibration.  Using either one of these instruments right out of the box will yield a variety of readings which is not good when you are trying to establish the salinity level of your aquarium.  Payam left with 20 gallons of my natural ocean water, salinity 33ppt, and when he got home and tested the water with his newly purchased Deep Six Hydrometer, it was reading over 40ppt!!  I knew this was incorrect.  Payam returned it to the store and bought another one.  Same problem.  Then he purchased yet another one! Finally–Getting closer! Third time’s a charm!

                         

Now, I know from personal experience because I have 2 of them myself and each one reads differently. The trick is to calibrate it with a known water source and then simply account for the difference each time you use it.  It’s no big problem to do.  The third one Payam purchased was a bit closer to an actual read and we agreed that he will bring it in for me to calibrate properly soon.  The point is, if you are continually having problems with your jellyfish eating, belling or pulsing, or just not thriving, you should first look to your salinity level.

Moon jellies perform better with a salinity level between 32-33ppt. Don’t worry if you go a bit below that because lower is better than higher. Just don’t alter the salinity level too much at one time but do it gradually over a few days.  And, never add distilled or RO water to your filter box as it will kill your beneficial bacteria, which are a marine (saltwater) species of bacteria and are also affected by salinity & pH.  Also, keep in mind:  depending on your geographic location and evaporation rate, you may need to check your salinity twice a week.

Here in Los Angeles we have very dry air and experience quite a lot of evaporation. But, again, it’s hard to know that if you have an instrument that is inaccurately reading your water.  Try taking your hydrometer to your local hi-end reef / fish store and see if they will calibrate it for you.  What you want to know is how many points off your hydrometer is – either too high or too low– and then just account for that each time you take a salinity reading.  For example, when using my plastic Deep Six hydrometer in a pinch, I know it reads 3 points too low, so I just add 3 points to whatever it is reading.  When I do have a chance to test it against my pricey refractometer, it is consistent with my adjusted plastic hydrometer read.